第1个回答 2010-12-29
目的 :探讨竹叶的水溶性提取液对小鼠的抗氧化及体外清除羟自由基 (·OH-)的作用。方法 :7月龄小鼠给予竹叶提取液 (8,12g·kg-1,ig) ,连续30d后 ,采用生化方法测定小鼠全血的超氧化歧化酶 (SOD)和谷胱甘肽过氧物酶 (GSH Px)活性 ,并检测小鼠心、肝、脑组织中脂褐质 (LF)、丙二醛 (MDA)和SOD含量。采用体外抗坏血酸体系产生·OH-的原理 ,观察竹叶提取液清除·OH-的作用。结果 :给予竹叶提取液的小鼠心、肝、脑组织和全血中SOD及GSH Px活力显著提高 ,而MDA和LF含量则明显下降。竹叶提取液亦能清除抗坏血酸体系产生的·OH-,且·OH-的清除率呈明显的量效相关性 (r =0 .976 )。结论 :竹叶提取液具有直接清除机体·OH-,且能提高组织中抗氧化酶的活性 ,抑制自由基损伤 ,防止脂质过氧化的作用。
【作者单位】：咸宁学院药学院药理教研室;咸宁学院药学院护理系;咸宁学院药学院药理教研室;咸宁学院药学院免疫教研室;咸宁学院药学院生化教研室 咸宁437100;湖北;咸宁437100;湖北;咸宁437100;湖北;咸宁437100;湖北;咸宁437100;湖北
【关键词】：竹叶;抗氧化;超氧化物歧化酶;氧自由基;羟自由基
【基金】：湖北省教育厅科研基金资助项目 (№ 2 0 0 3A0 0 8)
【分类号】：R285.5
【DOI】：cnki:ISSN:1009-2501.0.2004-05-025
【正文快照】：
竹叶是禾木科多年生常绿乔木或灌木植物淡竹的叶片,在我国具有悠久的食用和药用历史。竹叶性淡、微涩、寒,味甘、苦。近年来对竹叶提取物药用价值和保健功能的研究才刚起步,研究表明,竹叶中的总黄酮含量与银杏叶具有可比性,竹叶还含有大量的活性多糖成分和丰富的微量元素,具有
AIM: To study the antioxidant effects of extract solution of bamboo leaves in mice and the effects of scavenging hydroxyl free radical ( ·OH- ) in vitro. METHODS: The mice were treated with extract solution of bamboo leaves (8,12 g·kg -1 ,ig) daily for 30 d. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in blood and the contents of lipofuscin (LF),malondialdehyde (MDA) and SOD in liver,and brain and heart by biochemical methods were determined. ·OH- was induced by ascorbate-Cu 2+ reaction system,and the effect of extract solution of bamboo leaves on scavenging ·OH- was estimated by colorimetry. RESULTS: The extract solution of bamboo leaves could significantly increase the activities of SOD and GSH-Px in blood and the activities of SOD in liver,brain and heart in mice,and obviously reduce the contents of LF and MDA in mice tissues. The extract solution of bamboo leaves of 0.05 - 1.0 g·L -1 had markedly effects on scavenging ·OH- from 7.8 % to 87.7 % in dose-effect relationship (r= 0.976 ). CONCULSION: The extract solution of bamboo leaves can obviously inhibit damage of free radical and prevent lipid peroxidation. The mechanisms may associate with directly scavenging ·OH- ,and with increasing the activities of antioxidation enzymes in mice tissues.