帮忙翻译一下

Question

The advent of methodology for the chemoselective ligation
of peptide fragments has made proteins accessible targets for total chemical synthesis. Already, many proteins have been assembled from synthetic peptides using prior capture strategies.“Native chemical ligation”, the coupling of a peptide containing a C-terminal thioester with another peptide containing an N-terminal cysteine residue, has been the most widely applied of such strategies. “Expressed protein ligation” is an enabling extension of native chemical ligation in which the C-terminal thioester is accessed by using recombinant DNA technology.Although powerful, these methods are limited by their reliance on cysteine, which is the second least common residue.
Emerging strategies for protein assembly avoid the need for a cysteine residue at the ligation junction. The Staudinger ligation is one such strategy. In one version of the Staudinger ligation, a peptide with a C-terminal phosphinothioester is coupled with a second peptide having an N-terminal azido acid
to form a new peptide bond in a traceless manner, without residual atoms. The reaction occurs without detectable racemization. The Staudinger ligation has been used in the orthogonal assembly of a fully functional enzyme and for the site-specific immobilization of peptides and small molecules
on a surface.

Answer 1

方法论的出现为化学选择性结扎肽片段进行了蛋白质的化学全合成的可访问的目标。已，许多蛋白质已被组装合成肽使用之前捕获策略。“原生化学结扎”，一个含肽与另一个含肽的N-末端的半胱氨酸残基的C-末端的硫酯偶联，得到最广泛应用的策略。“表达的蛋白质结扎”是一个有利的扩展本地化学结扎的C-末端的硫酯是利用重组DNA技术访问。虽然强大，这些方法都依赖于半胱氨酸的限制，这是第二个最常见的残留物。蛋白质组装的新兴策略避免在结扎交界处的半胱氨酸残基的需要。施陶丁格连接是一个这样的策略。在一个版本的施陶丁格结扎，一个C-末端phosphinothioester肽是加上一个具有N端叠氮acidto形式在无痕的方式的一个新的肽键的二肽，无残余的原子。没有可检测到的外消旋化反应发生。施陶丁格连接已被应用在一个全功能的酶正交装配为定点固定化的肽和小分子表面追问

确定翻译为结扎是对的？？

追答

查了一下有道，是这样的，不过您自己可以考量考量

Answer 2

肽片段进行了蛋白质的化学全合成的可访问的目标。已，许多蛋白质已被组装合成肽使用之前捕获策略。“原生化学结扎”，一个含肽与另一个含肽的N-末端的半胱氨酸残基的C-末端的硫酯偶联，得到最广泛应用的策略。“表达的蛋白质结扎”是一个有利的扩展本地化学结扎的C-末端的硫酯是利用重组DNA技术访问。虽然强大，这些方法都依赖于半胱氨酸的限制，这是第二个最常见的残留物。
蛋白质组装的新兴策略避免在结扎交界处的半胱氨酸残基的需要。施陶丁格连接是一个这样的策略。在一个版本的施陶丁格结扎，一个C-末端phosphinothioester肽是加上一个具有一个N-末端二肽的叠氮酸
在无痕的方式形成一个新的肽键，无残余的原子。没有可检测到的外消旋化反应发生。施陶丁格连接已被应用在一个全功能的酶正交组件和用于肽和小分子定点固定化
在一个表面。